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Inampudi Sailaja ¹, Ivvala Anand Shaker ², Yedlapalli Keerthi Ratna ¹

Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S., Loni (M.S.), IN

Abstract

The aqueous extract of Piper betel L. and Nicotiania tabaum were studied for antioxidant activity on different in vitro models namely 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) assay, nitric oxide assay and trichloroacetic acid based reducing power method. Ascorbic acid was also evaluated for comparison. The extracts showed free radical scavenging property in the tested models. Piper betel L. showed 98.06% inhibition of DPPH at 1000 μg and its activity at 500 μg (i.e. 94.35%)was comparable to that of ascorbic acid at 30μg (93.58%). While the maximum percentage inhibition by Piper betel L. and Nicotiania tabaum in the nitric oxide model was found to be only 62.14% and 33.36%, respectively, the activity of 1024 μg of Nicotiania tabaum and 128μg of Piper betel L. of compares favorably with that of 30 μg ascorbic acid. Piper betel L. showed high reductive ability. This study demonstrates the higher anti oxidant activity is present in the leaves of Piper betel L. when compared to Nicotiania tabaum.

Keywords

Antioxidant, Piper betel L. and Nicotiania tabaum, Free Radicals, Reductive Ability.

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A. P. Sawant ¹, Ivvala Anand Shaker ²

Affiliations
1 Department of Biochemistry, Sinhgad Dental College and Hospital, Pune (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S., Loni, Ahmednagar (M.S.), IN

Abstract

The challange of diabetes during pregnancy is more delicate and greater than ever and health care professionals are facing it at all levels in the developing countries. There are no large-scale epidemiological studies available to define the magnitude of the problem in India. Indirectly, however, one can conjecture the potential magnitude. An urban diabetes survey in 1992 indicated a prevalence of 2.6% for diabetes mellitus and 9.9% for impaired glucose tolerance among reproductive women aged 20 to 34 years (Seshiah and Balaji, 2002); Interestingly, despite the highest pregnancy rates in India the only published report in this regard document 1.19% prevalence rate of diabetes among pregnant women (Kochupillai, 2002).

Keywords

Diabetic Pregnancies, Gestational Diabetes Millitus (GDM).

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K. Satya Narayana ¹, Sravanthi Koora ², Ivvala Anand Shaker ³, S. Saleem Basha ³, K. Suresh Babu ³

Affiliations
1 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
2 Department of Pharmacology, Chalmeda Anand Rao Institute of Medical Sciences, Karimnagar (A.P.), IN
3 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences and Research, Melmaruvathur (T.N.), IN

Abstract

Both diabetes and thyroid disorders involve dysfunction of the endocrine system. Almost one third of people with type 1 diabetes have been found to have thyroid disease. Since both diseases are parallel and influence one another. Any alteration in thyroid hormones may have further complication such as CAD. Low T₃ syndrome is well established in development of CAD. In the present study we assessed the circulating thyroid hormones and glycemic status among Diabetic population. T₃ levels are low in both Type I and II diabetes, and are directly correlated with poor glycemic control. Among the diabetic population women are worse affected than men. In conclusion, many diabetics showed a low T₃ levels, suggesting that there may be impairment in the extrathyroidal conversion of T₄ to T₃ (5' deiodinization) and in turn enhanced by a poor glycemic control. The study reveals that frequent checkup for thyroid hormones are compulsory to prevent further complications.

Keywords

Glycemic Status, Diabetes Mellitus, Thyroid Dysfunction, 5' Deiodinization, Low T₃ Syndrome, CAD.

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K. Satya Narayana ¹, Sravanthi Koora ², Inampudi Sailaja ³, Ivvala Anand Shaker ⁴

Affiliations
1 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
2 Department of Pharmacology, Chalmeda Anand Rao Institute of Medical Sciences, Bommakal Karimanagar (T.N.), IN
3 Department of Biotechnology, College of Agricultural Biotechnology, Loni (M.S.), IN
4 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN

Abstract

Free radicals especially the reactive oxygen species (ROS) implicated in etiology for over hundred diseases. Radiation is one of the major exogenous sources of free radicals in man and it has been proved that ionizing radiation produces ROS in biological system capable of destroying biomolecules. The objective of this study was to measure the serum antioxidant status level in health workers, to estimate the oxidative stress in red blood cells of radiographers by measuring the malondialdehyde (MDA) and percentage hemolysis of RBCs in comparison with controls, to determine the concentrations of antioxidants like vitamin E and catalase in the above groups, to determine the relationship between the levels of oxidative stress, antioxidants and the duration of occupational exposure in radiographers. 5ml of venous blood was collected in heparin. Plasma was used for estimation of vitamin E. The separated cells were used for the assay of oxidative stress parameters - malondialdehyde -oxidative hemolysis of RBS's (or) per cent of hemolysis of RBC, antioxidant status parameters- vitamin E and catalase. Present results show that the percentage of hemolysis of RBC was higher in, radiographers when compared with control group (p=0.0059). The RBC MDA (p=0.690), Catalase (p=0.050) and plasma vitamin 'E' levels (p=0.50) were higher in radiographers than in control group. Finally we can conclude that there is a mild increase in oxidative stress occurring as a result of chronic occupational exposure to low dose ionizing radiation.

Keywords

MDA, ROS, Vitamin E, Catalase and Oxidative Stress.

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Sailaja Inampudi ¹, Laxman Bhosale ¹, Anuradha Rohinikar ², Ivvala Anand Shaker ³, C. Gonjari Komal ⁴, Ajit Gangavane ⁵

Affiliations
1 Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), IN
2 Department of Biotechnology, Parul Institute of pplied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), IN
3 Department of Biochemistry, Parul Institute of Medical and Research, Parul University, Limda, Waghodia, Vadodara (Gujarat), IN
4 College of Agricultural Biotechnology, Loni, Pravaranagar (M.S.), IN
5 Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), IN

Abstract

An effective means for rapid multiplication of plant species of clonal origin is micro propagation. Various in vitro studies have been reported on different species of patchouli, one such method of propagation that can be usefully employed to produce relatively uniform plantlets in a short time is via in vitro culture but there is limited effort to study direct organogenesis, which supports cultivation by providing true type plants in large numbers.Therefore this study determines the effect of different concentrations of growth hormones on patchouli, micropropagation and rapid multiplication stages of patchouli plant within a short time with good results of micropropagation stages for regeneration of patchouli were successfully initiated.

Keywords

Patchouli, Alpha Naphthalene Acetic Acid (NAA), Indol Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin (kin).

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Inampudi Sailaja ¹, Ivvala Anand Shaker ², Yedlapalli Keerthi Ratna ¹

Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, Ahmednagar, (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S, Loni, Ahmednagar, (M.S.), IN

Abstract

Ocimum sanctum Linn, Ocimum bascilicum Linn, which have been normally used in traditional puja at every Indian Hindu home, was estimated by three different methods: ferric reducing antioxidant power (FRAP) assay, improved ABTS radical cation decolorization assay, and DPPH free radical scavenging assay. Additionally, their total phenolic contents were analyzed by Folin-Ciocalteau micro method. The result showed that Ocimum bascilicum seemed to be better sources of antioxidant compounds, followed by Ocimum sanctum, respectively. Total phenolic content analysis, were significantly related to those of FRAP, ABTS, DPPH.

Keywords

Antioxidant Status, Total Phenolic Content, O.bascilicum, O.sanctum.

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